Cosmetics & Alternative Toxicological Methods

Growing consumer sentiments and regulatory directives such as EU Cosmetics Directive (76/768/EEC) have enabled and expedited development of approved alternative test guidelines for toxicological methods. Vanta Bioscience is committed to the 3R’s (reduction, refinement and replacement) of animal based test methods by offering validated alternative toxicological methods.

Vanta Bioscience provide following tests:

In Vitro Toxicology Tests

Cytotoxicity
Dermal Irritation
Ocular Irritation

Alternative Toxicology Tests

Ocular Irritation (based on bovine cornea or chicken chorio-allantoic membrane)
Dermal Irritation (based on EPISKIN^TM)
Dermal Sensitization (based on EPISKIN^TM)

Cell viability assays (based on MTT) and cytokine release assays are being developed and validated to support the requirements of artificial human skin based studies. We have developed a roadmap for commissioning of alternative toxicological methods as approved by OECD.

Acute Oral Systemic Toxicity

Method		OECD Adoption
Up-and-down procedure for acute oral toxicity		OECD TG 436 (2009)

Dermal Phototoxicity

Method		OECD Adoption
3T3 NRU phototoxicity test for skin photo-irritation		OECD TG 432 (2004)

Dermal Corrosivity and Irritation

Method		OECD Adoption
EPISKIN™ in vitro human skin model skin corrosivity test		OECD TG 431 (2004)
EPISKIN™ in vitro human skin model skin irritation test		OECD TG 439 (2010)

Immunotoxicity: Allergic Contact Dermatitis

Method		OECD Adoption
LLNA: BrdU-ELISA for skin sensitization testing (a non radioisotopic LLNA test method)		OECD TG 442B (2010)

Ocular Corrosivity and Irritation

Method		OECD Adoption
Bovine corneal opacity and permeability test method to identify severe eye irritants/corrosives		OECD TG 437 (2009)
Isolated chicken eye test method to identify severe eye irritants/corrosives		OECD TG 438 (2009)

Pyrogen Testing

Method		OECD Adoption
Human whole blood/interleukin-1β in vitro pyrogen test		OECD TG 487 (2010) )

Genetic Toxicity

Method		OECD Adoption
In vitro mammalian cell micronucleus test		OECD TG 487 (2010)